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ArchivesReverse Transcriptase Polymerase Chain Reaction Pdf DownloadReverse Transcriptase Polymerase Chain Reaction Pdf Download > http://shorl.com/bubyvabytoly e52a6f0149 In addition to the qualitative study of gene expression, quantitative PCR can be utilized for quantification of RNA, in both relative and absolute terms,[5] by incorporating qPCR into the technique. First, Lin et al. PMID9888974. Once designed and synthesized, a known amount of the competitor RNA is added to experimental samples and is co-amplified with the target using RT-PCR. J. One precaution to note is that the internal control must be chosen so that it is not affected by the experimental treatment. However, since the dye does not discriminate the double-stranded DNA from the PCR products and those from the primer-dimers, overestimation of the target concentration is a common problem. "Quantification of mRNA using competitive RT-PCR with standard-curve methodology". PMID14664723. ^ Radoni A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A (January 2004). PMID22544908. II. RT-PCR can be used to diagnose genetic disease such as LeschNyhan syndrome. Appl. Microbiol. This section is written like a manual or guidebook. 7: 113. PMC1282026. 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